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Accelero provides assay development and validation capabilities to support your microbiological program. The biosafety level 2 laboratories are equipped in a way that microorganisms can be grown on solid agar plates, followed by cultivation of isolated colonies in liquid media.

 

Analytical services for biological samples.

Accelero can process and analyze potentially infectious samples like:

  • Sputum samples e.g. from pneumonia patients
  • Bronchoalveolar lavage (BAL)
  • Nasal swaps
  • Wound secretion

Human samples collected in the course of clinical trials will be handled, processed, and analyzed in compliance with the respective regulatory requirements (ICH GCP, Declaration of Helsinki, EMA GCLP reflection paper).

 

Colony forming units (CFU) and genomic DNA qPCR determination.

We quantify your microorganism of choise in biological samples: Either expressed as colony forming units (CFU) on solid agar plates, or determined as genomic DNA copy number in validated qPCR assays. Alternatively, we determine the live/dead cell count in Accelero´s qualified and validated flow cytometer.

 

Approval for targeted cultivation of microorganisms.

Accelero is approved for the cultivation of many common bacteria strains like:

  • Escherichia coli
  • Staphylococcus aureus, methicillin sensitive
  • Staphylococcus aureus, MRSA
  • Moraxella catarrhalis
  • Legionella pneumophila
  • Streptococcus pneumoniae
  • Mycoplasma pneumoniae
  • Haemophilus influenzae
  • Chlamydophilia pneumoniae

 

Cloning and expression capabilities.

You need to clone your construct in Escherichia coli or Pichia pastoris, but you do not have the resources to do it within your project time frames? Let us do the work for you. Accelero´s lab is approved for genetic engineering services like:

  • target sequence amplification
  • sequence verification
  • in-frame cloning for expression
  • transgene sequence characterization
  • transgene expression analysis

The nucleotide sequence analysis can be provided in cooperation with external partners, either as a standard service, or in compliance with Good Laboratory Practice (GLP) regulations. Please contact the Accelero team for additional information.

 

Purchasing the reference strain you need.

Accelero can purchase virtually any microbial strain of the BSL-2 level. The company is an accepted client for the following tissue culture collection providers:

  • American Tissue Culture Collection (ATCC), United States of America
  • National Collection of Type Cultures (NCTC), United Kingdom
  • German Collection of Microorganisms and Cell Cultures (DSMZ), Germany

 

If you are looking for microbiology services which have not been listed here, please do not hesitate to get in touch with us for additional information. Accelero is looking forward to help you!

 

Last update of this site: 02-NOV-2016 by Christian Lange